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We conducted a randomized, double-blind, controlled trial of adults who received intravenous rehydration treatment from April 2009 until July 2010, in an ED that had an annual census of 120,000 patient visits. The institutional review board approved the study, and informed consent was obtained from all participants.. To test our reasoning that the rat species and/or dosing could be responsible for the differences between studies [1,5], we simultaneously examined both parameters -- species and dosing. First, we used a rat species different from ZFR i.e., the commonly studied Sprague-Dawley (SD) albino rat. Second, in addition to using the same dose as previously (equivalent to a human dose of 12.5 mg/daily) in one group [1], we also increased the doses to levels comparable to human levels of 25 and 50 mg daily in two others. While not as high as in the previous study [5], still, these doses far exceed comparable doses recommended in humans..

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